5TR0
Lipoxygenase-1 (soybean) L754A mutant at 293K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-02-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.826561 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.452, 92.652, 100.722 |
Unit cell angles | 90.00, 93.39, 90.00 |
Refinement procedure
Resolution | 45.646 - 1.850 |
R-factor | 0.1414 |
Rwork | 0.140 |
R-free | 0.16950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pzw |
RMSD bond length | 0.006 |
RMSD bond angle | 0.771 |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10pre_2104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.650 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.100 | 0.838 |
Number of reflections | 142712 | |
<I/σ(I)> | 13.3 | 1.7 |
Completeness [%] | 99.7 | 96.6 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.994 | 0.535 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG-3350, Sodium Acetate |