5TQO
Lipoxygenase-1 (soybean) L546A/L754A mutant at 300K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 300 |
Detector technology | CCD |
Collection date | 2013-08-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.826561 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.610, 92.809, 101.267 |
Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
Resolution | 68.341 - 1.700 |
R-factor | 0.141 |
Rwork | 0.141 |
R-free | 0.16290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pzw |
RMSD bond length | 0.006 |
RMSD bond angle | 0.769 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10pre_2104) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.341 | 68.341 | 1.790 |
High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
Rmerge | 0.046 | 0.687 | |
Number of reflections | 184143 | ||
<I/σ(I)> | 7.3 | 7 | 1.1 |
Completeness [%] | 99.3 | 99.7 | 98.9 |
Redundancy | 3.7 | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG-3350, Sodium Acetate |