5TQO
Lipoxygenase-1 (soybean) L546A/L754A mutant at 300K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2013-08-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.826561 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.610, 92.809, 101.267 |
| Unit cell angles | 90.00, 94.11, 90.00 |
Refinement procedure
| Resolution | 68.341 - 1.700 |
| R-factor | 0.141 |
| Rwork | 0.141 |
| R-free | 0.16290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pzw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.769 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10pre_2104) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.341 | 68.341 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.046 | 0.687 | |
| Number of reflections | 184143 | ||
| <I/σ(I)> | 7.3 | 7 | 1.1 |
| Completeness [%] | 99.3 | 99.7 | 98.9 |
| Redundancy | 3.7 | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG-3350, Sodium Acetate |
