5TQB

Crystal structure of assembly chaperone of ribosomal protein L4 (Acl4) in complex with ribosomal protein L4 (RpL4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	121.000, 127.900, 42.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.949 - 2.400
R-factor	0.1909
Rwork	0.189
R-free	0.22470
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.735
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.400	2.400
Number of reflections	49797	8039
<I/σ(I)>	13.6	1.92
Completeness [%]	99.1	99.1
Redundancy	6.8	6.6
CC(1/2)	0.990	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	294	0.1 M BIS-TRIS (pH 5.5) 2 % (v/v) Tacsminate (pH 5.5) 13 % (w/v) PEG 3350