5TQ2

Crystal structure of amino terminal domains of the NMDA receptor subunit GluN1 and GluN2A in complex with zinc at GluN1 and GluN2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.282
Spacegroup name	P 21 21 21
Unit cell lengths	78.163, 117.826, 158.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.333 - 3.289
R-factor	0.2276
Rwork	0.224
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tpw
RMSD bond length	0.005
RMSD bond angle	0.753
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.333
High resolution limit [Å]	3.280
Rmerge	0.079
Number of reflections	41205
<I/σ(I)>	8.84
Completeness [%]	96.0
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.8M Ammonium sulfate, 2.5% Isopropanol