5TPP
Crystal Structure of DH270.5 (unliganded) from the DH270 Broadly Neutralizing N332-glycan Dependent Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.114, 68.798, 59.463 |
| Unit cell angles | 90.00, 113.21, 90.00 |
Refinement procedure
| Resolution | 44.956 - 1.850 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tyg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.236 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.956 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.128 | 0.087 | 0.560 |
| Rmeas | 0.153 | ||
| Rpim | 0.082 | ||
| Total number of observations | 126544 | ||
| Number of reflections | 42441 | ||
| <I/σ(I)> | 5 | ||
| Completeness [%] | 97.1 | 94.1 | 90.3 |
| Redundancy | 3 | 3.3 | 2.1 |
| CC(1/2) | 0.984 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 100 mM sodium citrate, pH 4.0 and 40% PEG 400 |
