5TPL
Crystal Structure of DH270.3 (unliganded) from the DH270 Broadly Neutralizing N332-glycan Dependent Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.245, 69.291, 115.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.712 - 2.500 |
| R-factor | 0.2172 |
| Rwork | 0.215 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tpp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.712 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.110 | 0.043 | 0.662 |
| Rmeas | 0.129 | 0.050 | 0.796 |
| Rpim | 0.065 | 0.024 | 0.429 |
| Total number of observations | 57856 | ||
| Number of reflections | 17644 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 97.2 | 90.6 | 99 |
| Redundancy | 3.3 | 3.2 | 3 |
| CC(1/2) | 0.997 | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100 mM sodium acetate, pH 4.5 and 1.4 M LiSO4 |
