5TPL

Crystal Structure of DH270.3 (unliganded) from the DH270 Broadly Neutralizing N332-glycan Dependent Lineage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97925
Spacegroup name	P 21 21 21
Unit cell lengths	63.245, 69.291, 115.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.712 - 2.500
R-factor	0.2172
Rwork	0.215
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tpp
RMSD bond length	0.010
RMSD bond angle	1.332
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.712	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.110	0.043	0.662
Rmeas	0.129	0.050	0.796
Rpim	0.065	0.024	0.429
Total number of observations	57856
Number of reflections	17644
<I/σ(I)>	7.1
Completeness [%]	97.2	90.6	99
Redundancy	3.3	3.2	3
CC(1/2)		0.997	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	100 mM sodium acetate, pH 4.5 and 1.4 M LiSO4