5TPK
Crystal Structure of Mouse Protocadherin-15 EC7-8 V875A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.796, 22.990, 70.741 |
| Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
| Resolution | 70.170 - 2.000 |
| R-factor | 0.19177 |
| Rwork | 0.190 |
| R-free | 0.22897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xhz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.170 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.045 | 0.141 |
| Number of reflections | 15280 | |
| <I/σ(I)> | 23.12 | 5.4 |
| Completeness [%] | 96.5 | 82.1 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2M AMMONIUM CHLORIDE, 10% PEG3350 |
