5TOR
Crystal structure of AAT D222T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-12-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.750, 124.130, 129.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.980 - 1.350 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.19280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5toq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.884 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.980 |
| High resolution limit [Å] | 1.350 |
| Number of reflections | 193193 |
| <I/σ(I)> | 14.64 |
| Completeness [%] | 98.0 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 40mM NaOAc, 2mM PLP, 8% PEG 6,000 |
