5TOD

Transmembrane protein 24 SMP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	83.395, 117.098, 148.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.550 - 2.960
R-factor	0.241
Rwork	0.239
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.504
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	148.130
High resolution limit [Å]	2.958
Rmerge	0.093
Number of reflections	30469
<I/σ(I)>	28.5
Completeness [%]	98.1
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100-200 mM MgCl2, 100 mM bis-tris propane pH 6.5-8.0, 45% PEG 200