5TO7

Structure of the TPR oligomerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-13
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	164.490, 51.190, 113.800
Unit cell angles	90.00, 129.32, 90.00

Refinement procedure

Resolution	44.019 - 2.600
R-factor	0.2386
Rwork	0.236
R-free	0.28090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tv5
RMSD bond length	0.005
RMSD bond angle	0.733
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.490	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.130	0.825
Number of reflections	28341
<I/σ(I)>	11.2	1.3
Completeness [%]	97.5	98.8
Redundancy	7.4	7.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.25	295	28% PEG1K, 0.1M HEPES (7.25)