5TNW
Phospholipase C gamma-1 C-terminal SH2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 33.360, 36.650, 38.780 |
Unit cell angles | 90.20, 101.89, 97.20 |
Refinement procedure
Resolution | 37.931 - 1.400 |
R-factor | 0.1884 |
Rwork | 0.187 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k44 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.722 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.931 | 37.931 | 1.440 |
High resolution limit [Å] | 1.366 | 4.320 | 1.370 |
Rmerge | 0.049 | 0.272 | |
Rmeas | 0.105 | ||
Rpim | 0.069 | ||
Total number of observations | 68865 | ||
Number of reflections | 34467 | ||
<I/σ(I)> | 5.7 | 11.5 | 2.4 |
Completeness [%] | 91.2 | 88.1 | 84.1 |
Redundancy | 2 | 2 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Imidazole (pH 7.0), 20% Jeffamine ED-2001 (pH 7.0) |