5TL5
COMPLEX BETWEEN HUMAN CD27 AND ANTIBODY M2177
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 274.200, 37.410, 61.080 |
| Unit cell angles | 90.00, 98.82, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.19725 |
| Rwork | 0.197 |
| R-free | 0.23178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iqw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.030 | 0.252 |
| Number of reflections | 56791 | |
| <I/σ(I)> | 20 | 4.1 |
| Completeness [%] | 97.2 | 96.5 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES PH 6.5, 17% PEG 4000, 0.2 M AMMONIUM CITRATE |
