5TKY
Crystal structure of the co-translational Hsp70 chaperone Ssb in the ATP-bound, open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.930, 128.240, 79.680 |
| Unit cell angles | 90.00, 93.22, 90.00 |
Refinement procedure
| Resolution | 46.430 - 2.600 |
| R-factor | 0.2297 |
| Rwork | 0.228 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b9q |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.542 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.430 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.103 | 1.260 |
| Number of reflections | 124469 | |
| <I/σ(I)> | 8.65 | 0.91 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 3.1 | 3.2 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG3350, 0.2 M ammonium phosphate |
