5TKW

1.35 Angstrom Resolution Crystal Structure of a Pullulanase-specific Type II Secretion System Integral Cytoplasmic Membrane Protein GspL (N-terminal fragment; residues 1-237) from Klebsiella pneumoniae.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 2
Unit cell lengths	63.901, 81.579, 40.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 1.350
R-factor	0.1478
Rwork	0.146
R-free	0.17629
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.490
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.069	0.733
Number of reflections	46935
<I/σ(I)>	45.9	2.03
Completeness [%]	99.4	95.4
Redundancy	6.7	3.8
CC(1/2)		0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Protein: 8.9 mg/ml, 0.5M Sodium chloride, 0.1M Tris HCl (pH 8.3);Screen: Classics II (A5), 2.0M Ammonium sulfate, 0.1M HEPES (pH 7.5);Cryo: 2.0M Ammonium sulfate, 25% Sucrose.