5TKO
RNA heptamer duplex with one 2'-5'-linkage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-05 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.541, 52.941, 27.732 |
Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
Resolution | 27.580 - 1.520 |
R-factor | 0.14744 |
Rwork | 0.144 |
R-free | 0.17727 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u37 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.708 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.083 | |
Number of reflections | 11869 | |
<I/σ(I)> | 17.8 | 1.95 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.1 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Precipitant: 40 mM sodium cacodylate pH5.5, 10% (v/v) MPD, 20 mM hexamine cobalt (III) chloride, 20mM MgCl2 Reservoir: 35% (v/v) MPD |