5TKE

Crystal Structure of Eukaryotic Hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	80
Detector technology	CCD
Collection date	2016-04-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	82.060, 96.576, 89.282
Unit cell angles	90.00, 115.04, 90.00

Refinement procedure

Resolution	44.249 - 2.481
R-factor	0.1838
Rwork	0.181
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cbj
RMSD bond length	0.010
RMSD bond angle	1.126
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.480
Number of reflections	44255
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	0.032 M ammonium citrate tribasic (pH 7.0), 0.02 M MES monohydrate, 0.016 M imidazole, 0.002 M zinc sulfate heptahydrate, 0.128 M potassium thiocyanate, 12.8% w/v polyethylene glycol 3,350, 3.2% w/v polyethylene glycol monomethyl ether 2,000, and 5% w/v polyethylene glycol monomethyl ether 550