5TK4
Crystal Structure of Uncharacterized Cupredoxin-like Domain Protein from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 43 |
| Unit cell lengths | 42.695, 42.695, 100.318 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.330 - 1.460 |
| R-factor | 0.12948 |
| Rwork | 0.128 |
| R-free | 0.16276 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hci |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.546 | |
| Number of reflections | 31013 | |
| <I/σ(I)> | 26.9 | 2.6 |
| Completeness [%] | 99.3 | 87.8 |
| Redundancy | 6.4 | 4.6 |
| CC(1/2) | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 289 | 34.3 % w/v PEG 4000, 150 mM Formate pH 4.0, 7 mM Nickel Chloride |
