5TK0

Lysozyme structure collected with 3D printed polymer mounts

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-24
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	77.886, 77.886, 37.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.471 - 1.795
R-factor	0.2119
Rwork	0.208
R-free	0.29140
RMSD bond length	0.006
RMSD bond angle	0.798
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.471
High resolution limit [Å]	1.795
Number of reflections	11074
<I/σ(I)>	16.2
Completeness [%]	99.7
Redundancy	6.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	5% NaCl 0.1M sodium acetate at pH 4.5