5TJW

Influenza A virus Nucleoprotein in Complex with Inhibitory Nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 21 3
Unit cell lengths	137.548, 137.548, 137.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.261 - 3.231
R-factor	0.2101
Rwork	0.204
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iqh 4krl
RMSD bond length	0.005
RMSD bond angle	0.633
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.260	3.340
High resolution limit [Å]	3.220	3.220
Rmerge	0.178
Number of reflections	14189
<I/σ(I)>	15.4	2.1
Completeness [%]	99.0	100
Redundancy	9.3	7.9
CC(1/2)	0.996	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.1 M sodium acetate, 1.5 M ammonium sulfate, 0.025% (v/v) dichloromethane