5TJS
Crystal structure of FBP aldolase from Toxoplasma gondii, native form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 110.218, 110.218, 54.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.968 - 1.780 |
| R-factor | 0.1572 |
| Rwork | 0.155 |
| R-free | 0.19810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qut |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
| Rmerge | 0.083 | 0.036 | |
| Rmeas | 0.083 | 0.038 | |
| Rpim | 0.024 | 0.010 | 0.477 |
| Total number of observations | 438181 | ||
| Number of reflections | 32711 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 99.1 | 99.8 | 87.8 |
| Redundancy | 13.4 | 13.3 | 8.7 |
| CC(1/2) | 0.999 | 0.516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 0.1M Sodium Acetate, 0.2M Lithium Sulfate, 3.5% PEG 8000, 10% PEG 1000 |
