Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0069 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 188.763, 188.763, 188.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.305 - 2.537 |
R-factor | 0.1806 |
Rwork | 0.179 |
R-free | 0.22300 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.925 |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.420 | 2.640 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.090 | 0.330 |
Number of reflections | 38400 | |
Completeness [%] | 99.4 | |
Redundancy | 33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 3.5-4.0 M sodium formate, 50 mM Tris, pH 7.5 |