5TIM

REFINED 1.83 ANGSTROMS STRUCTURE OF TRYPANOSOMAL TRIOSEPHOSPHATE ISOMERASE, CRYSTALLIZED IN THE PRESENCE OF 2.4 M-AMMONIUM SULPHATE. A COMPARISON WITH THE STRUCTURE OF THE TRYPANOSOMAL TRIOSEPHOSPHATE ISOMERASE-GLYCEROL-3-PHOSPHATE COMPLEX

Replaces:  2TIM

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	113.140, 97.690, 46.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.830
R-factor	0.183
RMSD bond length	0.020
RMSD bond angle	2.900
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	1.830 *
Rmerge	0.089 *
Total number of observations	122216 *
Number of reflections	38819 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	45 (mg/ml)
2	1	reservior	MOPS	0.2 (M)
3	1	reservoir	ammonium sulphate	2.4 (M)
4	1	reservoir	dithiothreitol	1 (mM)
5	1	reservoir	azide	1 (mM)
6	1	reservoir	EDTA	1 (mM)