5THB

Crystal structure of H10 hemagglutinin mutant (T193D-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	63.826, 241.286, 70.063
Unit cell angles	90.00, 111.43, 90.00

Refinement procedure

Resolution	33.055 - 2.410
R-factor	0.1829
Rwork	0.180
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tgo
RMSD bond length	0.011
RMSD bond angle	2.207
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.410	2.410
Number of reflections	74001
<I/σ(I)>	18.7	4.7
Completeness [%]	98.1
Redundancy	3.8	3.8
CC(1/2)		0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	0.2 M NaCl, 10% (w/v) PEG 8000, 0.1M CHES pH 9.5