5TH3
Restriction/modification system-Type II R.SwaI cleaved DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-14 |
| Detector | RIGAKU SATURN 70 |
| Wavelength(s) | 1.5400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.748, 57.072, 113.443 |
| Unit cell angles | 90.00, 107.60, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.330 |
| R-factor | 0.20051 |
| Rwork | 0.198 |
| R-free | 0.23891 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SwaI |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.984 | |
| Number of reflections | 56787 | |
| <I/σ(I)> | 17.8 | 1.35 |
| Completeness [%] | 98.2 | 85.9 |
| Redundancy | 7.1 | 4.7 |
| CC(1/2) | 0.678 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 24-28% PEG1000, 100 mM TrisHCl, 5 mM MgCl2 |
