5TGT
Crystal structure of glytamyl-tRNA synthetase GluRS from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-13 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.840, 138.660, 202.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.061 - 2.450 |
R-factor | 0.1853 |
Rwork | 0.183 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gri |
RMSD bond length | 0.008 |
RMSD bond angle | 1.067 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.061 | 2.510 | |
High resolution limit [Å] | 2.450 | 10.960 | 2.450 |
Rmerge | 0.060 | 0.024 | 0.496 |
Rmeas | 0.065 | 0.027 | 0.541 |
Total number of observations | 303206 | ||
Number of reflections | 49576 | 633 | 3564 |
<I/σ(I)> | 19.61 | 44.76 | 3.54 |
Completeness [%] | 99.9 | 96.1 | 100 |
CC(1/2) | 0.999 | 0.999 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | JCSG+ D9(271290d9): 170mM Ammonium Sulfate, 25.5% w/v PEG4000 15% v/v glycerol, 2mM BSI100156; dc; 24mg/mL; dkp0-3 |