5TGI

Structure of the SNX5 PX domain in complex with chlamydial protein IncE in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	60.711, 67.540, 88.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.151 - 1.980
R-factor	0.1847
Rwork	0.181
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.305
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.700	2.030
High resolution limit [Å]	1.980	1.980
Rmerge	0.104	0.525
Number of reflections	26075
<I/σ(I)>	12.4	3.4
Completeness [%]	100.0	100
Redundancy	6.9	6.1
CC(1/2)	0.997	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.2 M KSCN, 25% PEG 2K MME, 100 mM sodium acetate (pH 5.5)