5TGE
Thermus Phage P74-26 Large Terminase Nuclease Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.370, 71.370, 127.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.507 - 2.600 |
R-factor | 0.2211 |
Rwork | 0.220 |
R-free | 0.24540 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.504 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX ((1.10_2155)) |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.600 |
Number of reflections | 10480 |
<I/σ(I)> | 23.3 |
Completeness [%] | 98.0 |
Redundancy | 28 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 20 mg/mL protein mixed 2:1 with buffer containing 0.23M sodium phosphate monobasic/potassium phosphate dibasic pH 6.2 and 2.5M sodium chloride, and 4 mM dTMP |