5TGC

Structure of the hetero-trimer of Rtt102-Arp7/9 bound to ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-18
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	79.387, 87.982, 105.430
Unit cell angles	109.03, 104.64, 96.20

Refinement procedure

Resolution	39.366 - 3.245
R-factor	0.2779
Rwork	0.276
R-free	0.32190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i6m chains A B D
RMSD bond length	0.004
RMSD bond angle	0.940
Data scaling software	SAINT (v8.34a)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.366	3.420
High resolution limit [Å]	3.245	3.250
Rmerge	0.097	0.418
Number of reflections	40398
<I/σ(I)>	11.2
Completeness [%]	96.6	92
Redundancy	3.4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.5	0.17M Ammonium Sulfate, 20% PEG 3350, 17 mM EDTA