5TGB

Structure of chimeric 02-CB Fab, a VRC01-like germline antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	199.793, 64.638, 81.278
Unit cell angles	90.00, 101.49, 90.00

Refinement procedure

Resolution	47.213 - 2.741
R-factor	0.2505
Rwork	0.249
R-free	0.28560
RMSD bond length	0.003
RMSD bond angle	0.580
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.213
High resolution limit [Å]	2.741
Number of reflections	25516
<I/σ(I)>	4.8
Completeness [%]	95.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% isopropanol, 20% PEG 4000, 0.1 M Sodium citrate pH 5.6