5TG8
Crystal structure of H15 hemagglutinin from A/shearwater/WA/2576/1979 H15N9 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 3 |
Unit cell lengths | 115.991, 115.991, 128.451 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.388 - 3.100 |
R-factor | 0.2241 |
Rwork | 0.222 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n5j |
RMSD bond length | 0.014 |
RMSD bond angle | 2.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 34747 | |
<I/σ(I)> | 9.1 | 1.7 |
Completeness [%] | 98.7 | |
Redundancy | 4.1 | 3.1 |
CC(1/2) | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 20% (w/v) PEG 3500, 0.2M potassium nitrate pH 6.9. |