5TFW
Crystal structure of 10E8 Fab light chain mutant2 against the MPER region of the HIV-1 Env, in complex with T117v2 epitope scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2015-07-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 213.490, 63.410, 53.870 |
Unit cell angles | 90.00, 95.79, 90.00 |
Refinement procedure
Resolution | 47.235 - 2.168 |
R-factor | 0.1721 |
Rwork | 0.169 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g6f 3lf6 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.871 |
Data reduction software | XDS (VERSION November 3, 2014) |
Data scaling software | XSCALE (VERSION November 3, 2014 BUILT=20141118) |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.235 | 2.220 |
High resolution limit [Å] | 2.168 | 2.168 |
Rmerge | 0.635 | |
Number of reflections | 36138 | |
<I/σ(I)> | 15.5 | 2.1 |
Completeness [%] | 94.5 | 95.8 |
Redundancy | 4.2 | 4 |
CC(1/2) | 0.999 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.9 | 295.15 | 0.2 M Na thiocyanate, 20% PEG 3350, pH 6.9, 10% ethylene glycol |