5TFP

Crystal Structure of the SETDB2 Amino Terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97931
Spacegroup name	P 61 2 2
Unit cell lengths	69.474, 69.474, 120.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.740 - 2.000
R-factor	0.1629
Rwork	0.162
R-free	0.18100
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MLPHARE
Refinement software	PHENIX ((dev-1593))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.080	0.039	0.771
Number of reflections	12089
<I/σ(I)>	8.8
Completeness [%]	98.8	91.1	100
Redundancy	8	7.2	8
CC(1/2)		0.989	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.2 M ammonium tartrate (pH 6.0) and 20% PEG3350