5TFL

Crystal Structure of Mouse Cadherin-23 EC7+8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97921
Spacegroup name	P 41 3 2
Unit cell lengths	185.809, 185.809, 185.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	131.390 - 3.560
R-factor	0.19385
Rwork	0.192
R-free	0.22143
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Cadherin-23 (5I8D)
RMSD bond length	0.013
RMSD bond angle	1.365
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	131.390	3.610
High resolution limit [Å]	3.560	3.560
Rmerge	0.363	1.587
Number of reflections	13716
<I/σ(I)>	20	2.5
Completeness [%]	100.0	100
Redundancy	35.5	23.7
CC(1/2)		0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.7	277	0.1 M Sodium Acetate pH 4.7, 1.9 M Sodium Chloride, 30% PEG 400