5TEX
Pim-1 kinase in complex with a 7-azaindole
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2005-06-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 |
Unit cell lengths | 97.536, 97.536, 81.104 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.460 - 2.149 |
R-factor | 0.1842 |
Rwork | 0.181 |
R-free | 0.21670 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.003 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.460 |
High resolution limit [Å] | 2.149 |
Number of reflections | 23887 |
<I/σ(I)> | 3.67 |
Completeness [%] | 99.5 |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | imidazole 0,1M - NaAcetate 1M - pH6,5 |