5TET

TEV Cleaved Human ATP Citrate Lyase Bound to 4S Hydroxycitrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	55.210, 83.510, 193.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.080 - 2.200
R-factor	0.159
Rwork	0.156
R-free	0.21300
RMSD bond length	0.012
RMSD bond angle	1.003
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	53.090
High resolution limit [Å]	2.200
Number of reflections	45650
<I/σ(I)>	14
Completeness [%]	99.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	12.5% of P3350, 100 mM TrisHCL pH 7.5, 125 mM ammonium phosphate pH 7.5