5TEQ
C20S C293G MUTANT N-TERMINAL HUMAN ATP CITRATE LYASE BOUND TO CITRATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-18 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.110, 73.100, 131.120 |
| Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
| Resolution | 41.458 - 2.300 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24550 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.522 |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.080 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 79319 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 99.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 12.5% P3350, 100-200 mM MES pH 7, 125 mM ammonium citrate pH 4.5 |
