5TEE
Crystal structure of Gemin5 WD40 repeats in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.811, 124.407, 61.034 |
| Unit cell angles | 90.00, 119.49, 90.00 |
Refinement procedure
| Resolution | 48.470 - 1.650 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FFAS03/SCWRL models based on PDB entries 3ow8 2hes 3dm0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.594 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.710 | 48.710 | 1.680 |
| High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
| Rmerge | 0.091 | 0.033 | 1.015 |
| Rmeas | 0.107 | 0.039 | 1.179 |
| Rpim | 0.054 | 0.020 | 0.599 |
| Total number of observations | 355460 | 2137 | 17249 |
| Number of reflections | 93484 | ||
| <I/σ(I)> | 10.8 | 35.9 | 1.3 |
| Completeness [%] | 98.9 | 97.7 | 97.8 |
| Redundancy | 3.8 | 3.6 | 3.8 |
| CC(1/2) | 0.997 | 0.997 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 15% PEG4000, 0.2M ammonium acetate, 0.1M sodium citrate |
