5TEC

Crystal structure of the TIR domain from the Arabidopsis thaliana NLR protein SNC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	82.175, 82.175, 124.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.678 - 2.200
R-factor	0.1801
Rwork	0.178
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ozi
RMSD bond length	0.013
RMSD bond angle	1.286
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.680
High resolution limit [Å]	2.200
Number of reflections	22223
<I/σ(I)>	17.8
Completeness [%]	99.8
Redundancy	16.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	18%(w/v) PEG 3350, 9%(w/v) glycerol, 0.1 M MMT buffer pH 7.5