5TE9
Crystal structure of a response regulator receiver protein from Burkholderia phymatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.390, 103.840, 55.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.906 - 2.401 |
R-factor | 0.2086 |
Rwork | 0.201 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5BRI chain A |
RMSD bond length | 0.007 |
RMSD bond angle | 0.850 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (dev_2499) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.460 |
High resolution limit [Å] | 2.400 | 10.740 | 2.400 |
Rmerge | 0.062 | 0.037 | 0.567 |
Number of reflections | 6748 | ||
<I/σ(I)> | 19.51 | 41.91 | 3.75 |
Completeness [%] | 99.7 | 95.9 | 100 |
Redundancy | 3.5 | ||
CC(1/2) | 0.999 | 0.998 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | BuphA.00051.b.B1.PS37982 at 35 mg/mL against MCSG1 screen condition E4, 0.2 M Li2SO4, 0.1 M Tris pH 8.5, 25% PEG 3350 supplemented with 20% EG as cryo-protectant |