5TE7
Crystal Structure of Broadly Neutralizing VRC01-class Antibody N6 in Complex with HIV-1 Clade C Strain DU172.17 gp120 Core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.675, 65.547, 241.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.523 - 2.150 |
R-factor | 0.1912 |
Rwork | 0.190 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5te6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.539 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
Rmerge | 0.096 | 0.062 | 0.548 |
Rmeas | 0.110 | ||
Rpim | 0.052 | ||
Total number of observations | 223202 | ||
Number of reflections | 52871 | ||
<I/σ(I)> | 9.8 | ||
Completeness [%] | 96.2 | 95.3 | 90.2 |
Redundancy | 4.2 | 4.1 | 3.1 |
CC(1/2) | 0.993 | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 7 % w/v PEG 4000, 4 % (v/v) isopropanol, 2 % (v/v) benzamidine, 0.1 M HEPES, pH 7.5 |