5TE3
Crystal structure of Bos taurus opsin at 2.7 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97890 |
Spacegroup name | H 3 2 |
Unit cell lengths | 242.660, 242.660, 111.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.340 - 2.700 |
R-factor | 0.24541 |
Rwork | 0.245 |
R-free | 0.25378 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4j4q |
RMSD bond length | 0.004 |
RMSD bond angle | 0.866 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 34545 |
<I/σ(I)> | 20.9 |
Completeness [%] | 100.0 |
Redundancy | 22.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | contained 2.8-3.4 M ammonium sulfate in 0.05-0.1 M NaAcO buffer, pH 5.2-5.6 |