5TE3
Crystal structure of Bos taurus opsin at 2.7 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97890 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 242.660, 242.660, 111.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.340 - 2.700 |
| R-factor | 0.24541 |
| Rwork | 0.245 |
| R-free | 0.25378 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4j4q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.866 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 34545 |
| <I/σ(I)> | 20.9 |
| Completeness [%] | 100.0 |
| Redundancy | 22.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | contained 2.8-3.4 M ammonium sulfate in 0.05-0.1 M NaAcO buffer, pH 5.2-5.6 |
