5TDY

Structure of cofolded FliFc:FliGn complex from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97700
Spacegroup name	P 1 21 1
Unit cell lengths	49.180, 59.327, 51.722
Unit cell angles	90.00, 115.59, 90.00

Refinement procedure

Resolution	46.647 - 2.105
R-factor	0.174
Rwork	0.169
R-free	0.21730
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.880
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Rmerge	0.082
Number of reflections	15621
<I/σ(I)>	13.4
Completeness [%]	99.5
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Wild Type: 100 mM HEPES pH 7.5, 25% (w/v) PEG3000, 200 mM sodium chloride Selenomethionine: 100 mm imidazole pH 7.2, 30% (w/v) PEG8000, 130 mM sodium chloride
1	VAPOR DIFFUSION, HANGING DROP		298	Wild Type: 100 mM HEPES pH 7.5, 25% (w/v) PEG3000, 200 mM sodium chloride Selenomethionine: 100 mm imidazole pH 7.2, 30% (w/v) PEG8000, 130 mM sodium chloride