5TDK
RNA decamer duplex with eight 2'-5'-linkages
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 24.820, 47.020, 42.880 |
| Unit cell angles | 90.00, 96.52, 90.00 |
Refinement procedure
| Resolution | 18.700 - 1.430 |
| R-factor | 0.16984 |
| Rwork | 0.168 |
| R-free | 0.20585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4msb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.379 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.700 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.063 | 0.680 |
| Number of reflections | 8980 | |
| <I/σ(I)> | 13.3 | 2 |
| Completeness [%] | 98.6 | 91.9 |
| Redundancy | 3.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 40 mM sodium cacodylate buffer (pH 7.0), 10% (v/v) MPD (2-methyl-2,4-pentanediol), 12 mM spermine tetrahydrochloride, 40 mM LiCl, 80 mM SrCl2 and 20 mM MgCl2 |
