5TDG
Crystal structure of prefusion-stabilized bovine RSV F (DS-Cav1 variant: strain ATue51908)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.069, 127.033, 92.427 |
Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
Resolution | 92.210 - 2.650 |
R-factor | 0.2073 |
Rwork | 0.205 |
R-free | 0.24900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 92.210 |
High resolution limit [Å] | 2.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12% (w/v) PEG 3350, and 0.1M sodium acetate pH 5.5 |