5TDG
Crystal structure of prefusion-stabilized bovine RSV F (DS-Cav1 variant: strain ATue51908)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.069, 127.033, 92.427 |
| Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
| Resolution | 92.210 - 2.650 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.24900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 92.210 |
| High resolution limit [Å] | 2.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12% (w/v) PEG 3350, and 0.1M sodium acetate pH 5.5 |
