5TCV
ACC oxidase complex with substrate 1-aminocyclopropane-1-carboxylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 71.361, 107.140, 108.019 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.960 - 2.600 |
| R-factor | 0.23491 |
| Rwork | 0.232 |
| R-free | 0.29079 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wa6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.960 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 13101 | |
| <I/σ(I)> | 30 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 43.9 | 44.5 |
| CC(1/2) | 1.000 | 0.718 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 291 | sodium dihydrogenphosphate, lithium sulfate, pottasium hydrogen phosphate, CAPS pH 10.5 |
