5TCS
Crystal structure of a Dwarf Ndc80 Tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 169.381, 186.593, 122.039 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.750 - 2.831 |
| R-factor | 0.24 |
| Rwork | 0.239 |
| R-free | 0.26640 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.574 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.930 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.120 | 1.440 |
| Number of reflections | 45973 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 1.2 magnesium sulfate, 0.1 M ME pH 6.0 |
