5TCP
Near-atomic resolution cryo-EM structure of the periplasmic domains of PrgH and PrgK
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Energy Filter | Gatan GIF |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.3 |
| Number of Particle | 263900 |
| Software | EMAN2,RELION,FREALIGN,SerialEM,CTFFIND |
| Symmetry | C24 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
