5TC2
C-terminal domain of HIV-1 integrase, crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97858 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.139, 72.618, 33.893 |
| Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
| Resolution | 36.310 - 1.840 |
| R-factor | 0.219 |
| Rwork | 0.218 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ex4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.310 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.112 | 1.225 |
| Rmeas | 0.134 | 1.542 |
| Rpim | 0.072 | 0.921 |
| Number of reflections | 10034 | |
| <I/σ(I)> | 6 | 0.8 |
| Completeness [%] | 98.7 | 84.2 |
| Redundancy | 3.2 | 2.1 |
| CC(1/2) | 0.994 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 2.1M di-Sodium DL-malate |
