5TBZ
E. Coli RNA Polymerase complexed with NusG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 248.161, 313.781, 162.791 |
| Unit cell angles | 90.00, 130.23, 90.00 |
Refinement procedure
| Resolution | 162.190 - 7.000 |
| R-factor | 0.3332 |
| Rwork | 0.330 |
| R-free | 0.39460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 162.190 |
| High resolution limit [Å] | 7.000 |
| Number of reflections | 13735 |
| <I/σ(I)> | 2.6 |
| Completeness [%] | 100.0 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 0.1 M sodium dihydrogen phosphate pH 6.5, 12% (w/v) PEG 8000 |
