5TBX
hnRNP A18 RNA Recognition Motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.473, 56.149, 72.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.377 - 1.767 |
| R-factor | 0.2191 |
| Rwork | 0.217 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | hnRNP A1 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.086 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.430 | 72.430 | 1.800 |
| High resolution limit [Å] | 1.770 | 9.010 | 1.770 |
| Rmerge | 0.153 | 0.022 | 3.553 |
| Rmeas | 0.163 | 0.024 | 3.791 |
| Rpim | 0.055 | 0.009 | 1.299 |
| Total number of observations | 146455 | 1118 | 7344 |
| Number of reflections | 17190 | ||
| <I/σ(I)> | 13.5 | 56.4 | 0.7 |
| Completeness [%] | 99.8 | 99.8 | 96.2 |
| Redundancy | 8.5 | 6.6 | 7.9 |
| CC(1/2) | 0.999 | 1.000 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2 M sodium acetate trihydrate pH 8.0 in 20% w/v PEG 3350 from the PEG/Ion HT crystallization screen (Hampton Research, HR2-139). |
